Chang Fang-Rong, Hwang Tsong-Long, Yang Yu-Liang, Li Chia-En, Wu Chin-Chung, Issa Hamad H, Hsieh Wen-Bin, Wu Yang-Chang
Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
Planta Med. 2006 Nov;72(14):1344-7. doi: 10.1055/s-2006-951691. Epub 2006 Oct 4.
A new clerodane diterpenoid 16-hydroxycleroda-13-ene-15,16-olide-3-one (1) was isolated from the bark of Polyalthia longifolia var. pendula, along with 23 known compounds and phytosteroids. Among these compounds, 5 - 7, 10, and 24 were obtained for the first time from the family Annonaceae . The structures of the isolated compounds were determined by mass and spectroscopic analysis. The clerodane diterpenoids, 2 - 4, showed significant cytotoxicity towards Hep G2 and Hep 3B hepatoma cell lines. Furthermore, compound 5 exhibited potent anti-inflammatory activity towards formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B (fMLP/CB)-induced superoxide generation by neutrophils with IC50 = 0.60 +/- 0.09 microg/mL.
从垂叶暗罗（Polyalthia longifolia var. pendula）的树皮中分离出一种新的克罗烷二萜类化合物16-羟基克罗达-13-烯-15,16-内酯-3-酮（1），以及23种已知化合物和植物甾醇。在这些化合物中，5 - 7、10和24首次从番荔枝科植物中获得。通过质谱和光谱分析确定了分离出的化合物的结构。克罗烷二萜类化合物2 - 4对Hep G2和Hep 3B肝癌细胞系表现出显著的细胞毒性。此外，化合物5对甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸/细胞松弛素B（fMLP/CB）诱导的中性粒细胞超氧化物生成具有强大的抗炎活性，IC50 = 0.60 +/- 0.09微克/毫升。
