Szabó B, Tóth F D, Laczkó J, Kiss J, Ujhelyi E, Füst G, Hollán Z R
Institute of Microbiology, University Medical School, Debrecen, Hungary.
Acta Microbiol Hung. 1990;37(1):73-80.
The presence of virus in peripheral blood mononuclear cells of asymptomatic antibody positive haemophiliacs was detected by assaying for reverse transcriptase and confirmed by electron microscopy and immunofluorescence. HIV has been detected in 5 out of 7 individuals. In order to investigate strain variation, supernatant fluids of cultures were added to H9 and MT-4 cells. Virus was recovered in MT-4 cells in 3 cases, whereas the H9 cells only supported the replication of 2 strains. Viruses isolated from asymptomatic haemophiliacs have a narrower range of infectivity than HTLV-IIIB.
通过检测逆转录酶，在无症状抗体阳性血友病患者的外周血单核细胞中发现了病毒，并通过电子显微镜和免疫荧光进行了确认。在7名个体中的5名中检测到了HIV。为了研究毒株变异，将培养物的上清液加入到H9和MT - 4细胞中。3例在MT - 4细胞中回收了病毒，而H9细胞仅支持2种毒株的复制。从无症状血友病患者中分离出的病毒的感染范围比HTLV - IIIB更窄。
