Du M Q, Hutchinson W L, Johnson P J, Williams R
Liver Unit, Kings College Hospital, Denmark Hill, London, United Kingdom.
Cancer. 1991 Jan 15;67(2):476-80. doi: 10.1002/1097-0142(19910115)67:2<476::aid-cncr2820670226>3.0.co;2-8.
The reactivity of serum alpha-fetoprotein (AFP) from 20 patients with hepatocellular carcinoma (HCC) with immobilized lentil lectin was examined and found to be significantly greater (39% +/- 18%) than that of the same protein from seven patients with chronic liver disease (CLD, 11.2% +/- 3.3%), seven with fulminant hepatic failure (FHF, 10% +/- 8.4%), and eight normal pregnant women (4.1% +/- 2.7%). The reactivity with Concanavalin A (Con A) was also significantly greater for AFP from HCC patients (44.5% +/- 12.5%) than that from FHF patients (7.7% +/- 4%) and normal pregnant women (5.3% +/- 3.3%), but not from patients with CLD. The reactivity with lentil lectin permitted distinction between those with HCC (31.3% +/- 14.1%) and those with uncomplicated CLD (11.2% +/- 8.4%) even when the absolute levels of serum AFP were in the same range (80-400 ng/ml). Evaluation of the alterations by lectin binding methodology may be useful in overcoming problems associated with distinguishing between malignant and CLD, particularly at moderate serum AFP elevations.
检测了20例肝细胞癌（HCC）患者血清甲胎蛋白（AFP）与固定化扁豆凝集素的反应性，发现其反应性（39%±18%）显著高于7例慢性肝病（CLD）患者（11.2%±3.3%）、7例暴发性肝衰竭（FHF）患者（10%±8.4%）及8例正常孕妇（4.1%±2.7%）的相同蛋白。HCC患者AFP与伴刀豆球蛋白A（Con A）的反应性（44.5%±12.5%）也显著高于FHF患者（7.7%±4%）及正常孕妇（5.3%±3.3%），但与CLD患者无显著差异。即使血清AFP绝对水平处于相同范围（80 - 400 ng/ml），与扁豆凝集素的反应性也能区分HCC患者（31.3%±14.1%）和无并发症的CLD患者（11.2%±8.4%）。通过凝集素结合方法评估这些变化可能有助于克服区分恶性肿瘤和CLD相关的问题，尤其是在血清AFP中度升高时。
