Schönknecht G, Althoff G, Junge W
Biophysik, Fachbereich Biologie chemie, Universität Osnabrück, Germany.
FEBS Lett. 1990 Dec 17;277(1-2):65-8. doi: 10.1016/0014-5793(90)80810-6.
The size of the function unit of electrical events in thylakoid membranes was estimated by the minimum amount of gramicidin needed to discharge the flash light generated electrical potential difference. Early flash spectroscopic measurements have indicated that a single gramicidin dimer operates on an electrical function unit containing at least 2 x 10(5) chlorophyll molecules. In this study we present gramicidin titrations with more intact thylakoid preparations which revealed a more than hundred-fold greater lower limit for the electric unit size, namely 5 x 10(7) chlorophyll molecules. It is conceivable that the whole complicated thylakoid structure inside a chloroplast constitutes a single electric unit. It comprises more than 2 x 10(8) chlorophyll molecules in an area of more than 400 microns 2.
通过使闪光产生的电势差放电所需的短杆菌肽的最小量，估算了类囊体膜中电事件功能单元的大小。早期的闪光光谱测量表明，单个短杆菌肽二聚体作用于一个包含至少2×10⁵个叶绿素分子的电功能单元。在本研究中，我们用更完整的类囊体制剂进行了短杆菌肽滴定，结果显示电单元大小的下限要大一百多倍，即5×10⁷个叶绿素分子。可以想象，叶绿体内整个复杂的类囊体结构构成一个单一的电单元。它在超过400平方微米的面积内包含超过2×10⁸个叶绿素分子。
