Mauriello Emilia M F, Cangiano Giuseppina, Maurano Francesco, Saggese Virgilio, De Felice Maurilio, Rossi Mauro, Ricca Ezio
Department of Structural and Functional Biology, Federico II University of Napoli, via Cinthia, Complesso MSA, 80126 Napoli, Italy.
Vaccine. 2007 Jan 15;25(5):788-93. doi: 10.1016/j.vaccine.2006.09.013. Epub 2006 Sep 22.
Bacillus subtilis spores displaying the tetanus toxin fragment C (TTFC) on their surface have been previously shown to induce the production of specific IgG and secretory IgA in mice immunized through the oral or nasal route. Aim of this study was to analyze whether these spores were also able to induce cellular immunity, and whether such immune response was dependent on spore germination in the animal gastro-intestinal tract (GIT). We first developed a germination defective strain of B. subtilis unable to produce viable cells inside the mouse GIT. Germination-defective and congenic wild-type spores both expressing TTFC on their surface were then used to orally immunize Balb/C mice. Both types of spores induced spleen and mesenteric lymph nodes cell proliferation as well as production of IFNgamma but not of IL-4 and IL-10 in both districts. Our results indicate that recombinant spores preferentially induce a strong cell-mediated immune response with a Th1 phenotype, independently from their ability to germinate in the GIT.
先前已表明，表面展示破伤风毒素片段C（TTFC）的枯草芽孢杆菌孢子，经口服或鼻内途径免疫小鼠后，可诱导产生特异性IgG和分泌型IgA。本研究的目的是分析这些孢子是否也能诱导细胞免疫，以及这种免疫反应是否依赖于孢子在动物胃肠道（GIT）中的萌发。我们首先构建了一种枯草芽孢杆菌的萌发缺陷菌株，该菌株在小鼠GIT内无法产生活细胞。然后，使用表面均表达TTFC的萌发缺陷型和同基因野生型孢子对Balb/C小鼠进行口服免疫。两种类型的孢子均诱导了脾脏和肠系膜淋巴结细胞增殖，以及两个部位的IFNγ产生，但未诱导IL-4和IL-10产生。我们的结果表明，重组孢子优先诱导具有Th1表型的强烈细胞介导免疫反应，而与它们在GIT中萌发的能力无关。
