Zhang Ying, Mo Ruo, Sun Sheng, Cui Zhanding, Liang Bo, Li Entao, Wang Tiecheng, Feng Ye, Yang Songtao, Yan Feihu, Zhao Yongkun, Xia Xianzhu
Northeast Forestry University College of Wildlife and Protected Area, Harbin, China.
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China.
Front Microbiol. 2023 Feb 9;14:1126533. doi: 10.3389/fmicb.2023.1126533. eCollection 2023.
Rabies is a worldwide epidemic that poses a serious threat to global public health. At present, rabies in domestic dogs, cats, and some pets can be effectively prevented and controlled by intramuscular injection of rabies vaccine. But for some inaccessible animals, especially stray dogs, and wild animals, it is difficult to prevent with intramuscular injection. Therefore, it is necessary to develop a safe and effective oral rabies vaccine.
We constructed recombinant () expressing two different strains of rabies virus G protein, named CotG-E-G and CotG-C-G, immunogenicity was studied in mice.
The results showed that CotG-E-G and CotG-C-G could significantly increase the specific SIgA titers in feces, serum IgG titers, and neutralizing antibodies. ELISpot experiments showed that CotG-E-G and CotG-C-G could also induce Th1 and Th2 to mediate the secretion of immune-related IFN-γ and IL-4. Collectively, our results suggested that recombinant CotG-E-G and CotG-C-G have excellent immunogenicity and are expected to be novel oral vaccine candidates for the prevention and control of wild animal rabies.
狂犬病是一种全球性流行病，对全球公共卫生构成严重威胁。目前，通过肌肉注射狂犬病疫苗可有效预防和控制家犬、猫及一些宠物的狂犬病。但对于一些难以接近的动物，尤其是流浪狗和野生动物，肌肉注射预防很困难。因此，有必要研发一种安全有效的口服狂犬病疫苗。
我们构建了表达两种不同狂犬病病毒G蛋白毒株的重组体（），命名为CotG-E-G和CotG-C-G，并在小鼠中研究了其免疫原性。
结果表明，CotG-E-G和CotG-C-G可显著提高粪便中特异性SIgA滴度、血清IgG滴度和中和抗体。ELISpot实验表明，CotG-E-G和CotG-C-G还可诱导Th1和Th2介导分泌免疫相关的IFN-γ和IL-4。总体而言，我们的结果表明重组体CotG-E-G和CotG-C-G具有优异的免疫原性，有望成为预防和控制野生动物狂犬病的新型口服疫苗候选物。
