Yago Tadayuki, Zarnitsyna Veronika I, Klopocki Arkadiusz G, McEver Rodger P, Zhu Cheng
Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA.
Biophys J. 2007 Jan 1;92(1):330-42. doi: 10.1529/biophysj.106.090969. Epub 2006 Oct 6.
Flow-enhanced cell adhesion is a counterintuitive phenomenon that has been observed in several biological systems. Flow augments L-selectin-dependent adhesion by increasing the initial tethering of leukocytes to vascular surfaces and by strengthening their subsequent rolling interactions. Tethering or rolling might be influenced by physical factors that affect the formation or dissociation of selectin-ligand bonds. We recently demonstrated that flow enhanced rolling of L-selectin-bearing microspheres or neutrophils on P-selectin glycoprotein ligand-1 by force decreased bond dissociation. Here, we show that flow augmented tethering of these microspheres or cells to P-selectin glycoprotein ligand-1 by three transport mechanisms that increased bond formation: sliding of the sphere bottom on the surface, Brownian motion, and molecular diffusion. These results elucidate the mechanisms for flow-enhanced tethering through L-selectin.
流动增强的细胞黏附是一种在多个生物系统中观察到的与直觉相悖的现象。流动通过增加白细胞与血管表面的初始拴系以及加强它们随后的滚动相互作用，增强了L-选择素依赖性黏附。拴系或滚动可能受影响选择素-配体键形成或解离的物理因素影响。我们最近证明，流动通过力降低键解离，增强了携带L-选择素的微球或中性粒细胞在P-选择素糖蛋白配体-1上的滚动。在此，我们表明流动通过三种增加键形成的转运机制增强了这些微球或细胞与P-选择素糖蛋白配体-1的拴系：球体底部在表面的滑动、布朗运动和分子扩散。这些结果阐明了通过L-选择素实现流动增强拴系的机制。
