Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
J Mar Biotechnol. 1998 Dec;6(4):249-254.
Randomly amplified polymorphic DNA (RAPD) analysis was used to examine genetic variation in wild black tiger shrimp, Penaeus monodon. Specimens were collected from five geographically separated locations (Satun-Trang, Phangnga, and Medan in the Andaman Sea and Chumphon and Trad in the Gulf of Thailand). A total of 100 P. monodon individuals were investigated using seven arbitrarily selected primers. Fifty-eight (72.5%) of eighty reproducible RAPD fragments ranging in size from 200 to 2200 bp were polymorphic. The percentages of polymorphic bands of the five geographic populations investigated varied from 51.5 to 57.7%. The genetic distance between populations and UPGMA dendrograms indicated that the Medan population was genetically different from Thai P. monodon (Dij = 14.976%). Within Thailand, the Satun-Trang P. monodon was separated from the remaining geographic populations with a genetic distance of 2.632%. RAPD analysis in the present study yielded a total of 252 genotypes. A Monte Carlo analysis illustrated geographic heterogeneity in genotype frequencies within this species, suggesting that genetic population structure does exist in this taxon (P < 0.001 for all primers). Signficant differences in genotype frequencies between Thai and Indonesian (Medan) P. monodon were observed (P < 0.0001). Within Thailand, the Andaman Sea P. monodon was significantly different from that of the Gulf of Thailand (P values between 0.0000 and 0.0387), indicating population differentiation between P. monodon from these two main fishery regions of Thailand.
随机扩增多态性DNA（RAPD）分析被用于检测野生黑虎虾（斑节对虾）的遗传变异。样本采集自五个地理上相互分隔的地点（安达曼海的沙敦 - 董里、攀牙和棉兰，以及泰国湾的春蓬和达叻）。使用七个任意选择的引物对总共100只斑节对虾个体进行了研究。在大小从200到2200 bp的80个可重复的RAPD片段中，有58个（72.5%）是多态性的。所调查的五个地理种群的多态性条带百分比在51.5%至57.7%之间变化。种群之间的遗传距离和UPGMA聚类图表明，棉兰种群在遗传上与泰国斑节对虾不同（Dij = 14.976%）。在泰国境内，沙敦 - 董里的斑节对虾与其他地理种群分开，遗传距离为2.632%。本研究中的RAPD分析总共产生了252种基因型。蒙特卡罗分析表明该物种内基因型频率存在地理异质性，这表明该分类单元中确实存在遗传种群结构（所有引物的P < 0.001）。观察到泰国和印度尼西亚（棉兰）斑节对虾之间基因型频率存在显著差异（P < 0.0001）。在泰国境内，安达曼海的斑节对虾与泰国湾的斑节对虾有显著差异（P值在0.0000至0.0387之间），这表明泰国这两个主要渔业区域的斑节对虾之间存在种群分化。
