Li Qing, Morimoto Kanehisa, Nakadai Ari, Qu Tianli, Matsushima Hiroki, Katsumata Masao, Shimizu Takako, Inagaki Hirofumi, Hirata Yukiyo, Hirata Kimiko, Kawada Tomoyuki, Lu Yuquan, Nakayama Kunio, Krensky Alan M
Department of Hygiene and Public Health, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan.
Prev Med. 2007 Feb;44(2):117-23. doi: 10.1016/j.ypmed.2006.08.017. Epub 2006 Oct 9.
It is well documented that natural killer (NK) cells provide host defense against tumors and viruses. We previously showed that lifestyle affects human NK and LAK activities. In order to explore the underlying mechanism, we investigated the effect of lifestyle on intracellular perforin, granulysin, and granzymes A/B in peripheral blood lymphocytes (PBL).
114 healthy male subjects, aged 20-59 years, from a large company in Osaka, Japan were selected with informed consent. The subjects were divided into groups reporting good, moderate, and poor lifestyles according to their responses on a questionnaire regarding eight health practices (cigarette smoking, alcohol consumption, sleeping hours, working hours, physical exercise, eating breakfast, balanced nutrition, and mental stress). Peripheral blood was taken, and numbers of NK, T, perforin, granulysin, and granzymes A/B-expressing cells in PBL were measured by flow cytometry.
Subjects with good or moderate lifestyle showed significantly higher numbers of NK, and perforin, granulysin, and granzymes A/B-expressing cells and a significantly lower number of T cells in PBL than subjects with poor lifestyle. Among the eight health practices, cigarette smoking, physical exercise, eating breakfast, and balanced nutrition significantly affect the numbers of NK, T cells, perforin, granulysin, and/or granzymes A/B-expressing cells, and alcohol consumption significantly affects the number of granzyme A-expressing cells. On the other hand, mental stress, sleeping, and working hours had no effect on those parameters.
Taken together, these findings indicate that poor lifestyle significantly decreases the numbers of NK, perforin, granulysin, and granzymes A/B-expressing cells in PBL.
充分的文献记载表明，自然杀伤（NK）细胞为宿主提供针对肿瘤和病毒的防御。我们之前表明生活方式会影响人类NK和LAK活性。为了探究潜在机制，我们研究了生活方式对外周血淋巴细胞（PBL）中细胞内穿孔素、颗粒溶素和颗粒酶A/B的影响。
在获得知情同意的情况下，从日本大阪一家大公司选取了114名年龄在20 - 59岁的健康男性受试者。根据他们对一份关于八项健康行为（吸烟、饮酒、睡眠时间、工作时间、体育锻炼、吃早餐、营养均衡和精神压力）的问卷回答，将受试者分为生活方式良好、中等和较差的组。采集外周血，通过流式细胞术测量PBL中NK、T、穿孔素、颗粒溶素和表达颗粒酶A/B的细胞数量。
与生活方式较差的受试者相比，生活方式良好或中等的受试者PBL中NK、穿孔素、颗粒溶素和表达颗粒酶A/B的细胞数量显著更高，而T细胞数量显著更低。在八项健康行为中，吸烟、体育锻炼、吃早餐和营养均衡显著影响NK、T细胞、穿孔素、颗粒溶素和/或表达颗粒酶A/B的细胞数量，饮酒显著影响表达颗粒酶A的细胞数量。另一方面，精神压力、睡眠时间和工作时间对这些参数没有影响。
综上所述，这些发现表明不良生活方式会显著减少PBL中NK、穿孔素、颗粒溶素和表达颗粒酶A/B的细胞数量。
