Rosztóczy I, Content J
Institute of Microbiology, A. Szent-Györgyi Medical University, Szeged, Hungary.
Acta Virol. 1990 May;34(3):288-90.
MuIFN-alpha/beta at a concentration of 0.5 IU/ml exerted both antiviral and priming effects in the B, but not in the M subline of L929 cells. This concentration of IFN did not affect the small size 2-5A synthetase mRNA levels in either sublines, as determined by a Northern blot technique. Pretreatment with 5 or 50 IU/ml IFN resulted in more pronounced antiviral and priming effects in the L929B than in the L929M cells. However, the enhancements of the 2-5A synthetase mRNA levels due to the pretreating IFN dose were similar in both sublines.
浓度为0.5国际单位/毫升的鼠干扰素α/β在L929细胞的B亚系中发挥了抗病毒和启动作用，但在M亚系中未发挥作用。通过Northern印迹技术测定，该浓度的干扰素对两个亚系中低分子量2-5A合成酶的mRNA水平均无影响。用5或50国际单位/毫升干扰素预处理后，L929B细胞比L929M细胞产生更显著的抗病毒和启动作用。然而，两个亚系中由于预处理干扰素剂量导致的2-5A合成酶mRNA水平的提高是相似的。
