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A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification.

作者信息

von Weizsäcker F, Labeit S, Koch H K, Oehlert W, Gerok W, Blum H E

机构信息

Massachusetts General Hospital, Harvard Medical School, Charlestown.

出版信息

Biochem Biophys Res Commun. 1991 Jan 15;174(1):176-80. doi: 10.1016/0006-291x(91)90502-x.

DOI:10.1016/0006-291x(91)90502-x
PMID:1703409
Abstract

A simple and rapid method for the extraction, reverse transcription and PCR amplification of RNA from formalin-fixed, paraffin-embedded tissue is described. The procedure can be completed within 24 hours. In a first application of this method we detect human albumin mRNA in liver tissue, demonstrating the feasibility to retrospectively analyze gene expression and RNA viruses in fixed tissue.

摘要

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1
A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification.
Biochem Biophys Res Commun. 1991 Jan 15;174(1):176-80. doi: 10.1016/0006-291x(91)90502-x.
2
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8
Effect of fixation on the amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction.固定对通过聚合酶链反应从石蜡包埋材料中扩增核酸的影响。
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Nucleic Acids Res. 1995 Aug 11;23(15):3071-2. doi: 10.1093/nar/23.15.3071.

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