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通过荧光原位杂交技术检测新生儿肠道中双歧杆菌的存在情况。

Occurrence of Bifidobacterium in the intestine of newborns by fluorescence in situ hybridization.

作者信息

Bezirtzoglou E, Maipa V, Chotoura N, Apazidou E, Tsiotsias A, Voidarou C, Kostakis D, Alexopoulos A

机构信息

Department of Food Science and Technology, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece.

出版信息

Comp Immunol Microbiol Infect Dis. 2006 Nov;29(5-6):345-52. doi: 10.1016/j.cimid.2006.09.002. Epub 2006 Oct 10.

DOI:10.1016/j.cimid.2006.09.002
PMID:17034855
Abstract

Colonization by Bifidobacterium occurs generally within 4 days of life. The new method FISH has been applied for molecular detection of Bifidobacteria. The study was carried out on 26 healthy newborns delivered by vaginal delivery. Breast-fed infants harbor a gastrointestinal flora characterized by an increased concentration of Bifidobacterium cells (by a factor of 1.75). In artificial alimentation, some infants either did not harbor any Bifidobacterium or showed lower numbers of Bifidobacterium. Moreover, male newborns show higher numbers of Bifidobacterium, but in both sexes the predominance of Bifidobacterium is evident after maternal alimentation.

摘要

双歧杆菌的定殖通常发生在出生后的4天内。新的荧光原位杂交(FISH)方法已被应用于双歧杆菌的分子检测。该研究对26名经阴道分娩的健康新生儿进行。母乳喂养的婴儿胃肠道菌群的特点是双歧杆菌细胞浓度增加(增加了1.75倍)。在人工喂养的情况下,一些婴儿要么没有双歧杆菌定殖,要么双歧杆菌数量较少。此外,男性新生儿的双歧杆菌数量较多,但在两性中,经母体喂养后双歧杆菌占优势的情况都很明显。

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