Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030, China.
World J Microbiol Biotechnol. 2013 Dec;29(12):2365-72. doi: 10.1007/s11274-013-1404-3. Epub 2013 Jun 23.
The gastrointestinal microbiota plays a crucial role in the health and disease of the host through its impact on nutrition. Gut microbial composition is related to different diets, but an association of microbiota with different diets in infant has not yet been shown. In this work, we compared the fecal microbiota of breast-fed (BF) and formula-fed infants (FF). By using Illumina high-throughput sequencing and biochemical analyses, we found differences in gut microbiota between the two groups. BF infants showed a significant enrichment of Actinobacteria and Firmicutes and depletion of Proteobacteria (P < 0.05), the abundance of Bacteroidetes in the two groups was very low (P > 0.05). Enterobacteriaceae (Proteobacteria) were the dominant bacteria in FF infant fecal microbiota, and Veillonellaceae (Firmicutes) and Enterobacteriaceae (Proteobacteria) were the dominant bacteria in the BF infant fecal microbiota. The number of genera (percentage of sequences >0.1 %) in BF and FF infants was 17 and 15 respectively, and Streptococcus was the dominant bacterial genus in both groups.
肠道微生物群通过其对营养的影响在宿主的健康和疾病中发挥着关键作用。肠道微生物组成与不同的饮食有关，但微生物群与婴儿不同饮食的关联尚未得到证实。在这项工作中，我们比较了母乳喂养（BF）和配方奶喂养（FF）婴儿的粪便微生物群。通过使用 Illumina 高通量测序和生化分析，我们发现两组之间的肠道微生物群存在差异。BF 组婴儿的放线菌和厚壁菌明显丰富，变形菌减少（P < 0.05），两组中拟杆菌的丰度非常低（P > 0.05）。肠杆菌科（变形菌）是 FF 婴儿粪便微生物群中的优势菌，而韦荣球菌科（厚壁菌）和肠杆菌科（变形菌）是 BF 婴儿粪便微生物群中的优势菌。BF 和 FF 婴儿的菌属数量（序列百分比> 0.1%）分别为 17 和 15，两组的优势细菌属均为链球菌属。
