Pediatrics Institute, Key Laboratory of Neonatal Diseases, Ministry of Health, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China.
Department of Pediatric Healthcare, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai, 201102, China.
World J Pediatr. 2016 May;12(2):177-82. doi: 10.1007/s12519-015-0025-3. Epub 2015 Apr 6.
Intestinal microbiotas are thought to be the most important source of maturational stimuli to the development of the immune system. However, few studies have focused on the development of T helper (Th) 1 immune response and antibody response to vaccinations in healthy infants, especially in a large cohort. Through this randomized, double-blind control trial, we investigated the effects of Bifidobacterium longum BB536 (BB536) supplementation on intestinal microbiota composition and the immune response in term infants.
In total, 300 healthy newborns were recruited, randomized and fed formula either supplemented with BB536 or with no supplementation. Stool samples were analyzed at months 2, 4 and 11. The representative cytokine for Th1 [interferon-γ (IFN-γ)] and Th2 [interleukin-4 (IL-4)] secretion cells were measured using enzyme-linked immunospot assay at 4 and 7 months of age. The antibody response to vaccines was measured at months 7 and 11.
A total of 264 infants completed the study. The amount of bifidobacteria and the bifidobacteria/ Enterobacteriaceae ratio (B/E) were significantly higher in the BB536 supplementation group at months 2 and 4. The number of IFN-γ secretion cells and the ratio of IFN-γ/IL-4 secretion cells were increased in the BB536 supplementation group at 7 months. Moreover, the higher value of B/E in the early stages seems to be related to the increased Th1 response. No difference was observed between groups in the antibody response after vaccination.
BB536 has positive effects on establishing a healthy intestinal microbiota early in life, and it also plays an important role in improving the Th1 immune response.
肠道微生物群被认为是对免疫系统发育成熟的最重要刺激源。然而，很少有研究关注健康婴儿中 T 辅助（Th）1 免疫反应和疫苗接种抗体反应的发展，特别是在大样本队列中。通过这项随机、双盲对照试验，我们研究了长双歧杆菌 BB536（BB536）补充对足月婴儿肠道微生物群组成和免疫反应的影响。
共招募了 300 名健康新生儿，随机分为 BB536 补充组和无补充组，分别喂养配方奶。在 2、4 和 11 个月时分析粪便样本。在 4 个月和 7 个月时使用酶联免疫斑点法测量 Th1（干扰素-γ（IFN-γ））和 Th2（白细胞介素-4（IL-4））分泌细胞的代表性细胞因子。在 7 个月和 11 个月时测量疫苗抗体反应。
共有 264 名婴儿完成了研究。在 2 个月和 4 个月时，BB536 补充组双歧杆菌数量和双歧杆菌/肠杆菌比值（B/E）显著升高。在 7 个月时，BB536 补充组 IFN-γ 分泌细胞数量和 IFN-γ/IL-4 分泌细胞比值增加。此外，早期较高的 B/E 值似乎与 Th1 反应的增加有关。接种疫苗后，两组之间的抗体反应无差异。
BB536 对早期建立健康肠道微生物群有积极作用，对改善 Th1 免疫反应也有重要作用。
