Gore Claudia, Munro Karen, Lay Christophe, Bibiloni Rodrigo, Morris Julie, Woodcock Ashley, Custovic Adnan, Tannock Gerald W
North West Lung Research Center, Wythenshawe Hospital, Manchester, United Kingdom.
J Allergy Clin Immunol. 2008 Jan;121(1):135-40. doi: 10.1016/j.jaci.2007.07.061. Epub 2007 Sep 27.
Exposure to specific bacterial bowel commensals may increase/reduce the risk of atopic diseases.
To compare fecal bacterial communities of young infants with/without eczema.
Nested case-control study. Infants age 3 to 6 months with eczema (cases, n = 37) and without (controls, n = 24) were matched for sex, age, feeding (breast/bottle/mixed/solids), ethnicity. Information was collected on maternal/infant antibiotic exposure, feeding, gastrointestinal symptoms, family history of allergy. Eczema severity scoring was used (Severity Scoring of Atopic Dermatitis index). Samples were taken for determination of allergen-specific serum IgE (cases) and urinary/fecal eosinophilic protein X. Gastrointestinal permeability was measured. The compositions of fecal bacterial communities were analyzed (culture-independent, nucleic acid-based analyses).
There was no difference in overall profiles of fecal bacterial communities between cases and controls. Family history of allergy increased likelihood of bifidobacteria detection (history, 86%; no history, 56%; P = .047); breast-fed infants were more likely to harbor Bifidobacterium bifidum (odds ratio, 5.19; 95% CI, 1.47-18.36; P = .01). Bifidobacterium pseudocatenulatum was detected more commonly in feces of non-breast-fed children (odds ratio, 5.6; 95% CI, 1.3-24.3; P = .02) and children with eczema (eczema, 26%; no eczema, 4%; P = .04). There were no significant associations between clinical measurements and detection of B pseudocatenulatum.
Presence of B pseudocatenulatum in feces was associated with eczema and with exclusive formula-feeding; B bifidum was associated with breast-feeding.
接触特定的肠道共生细菌可能会增加/降低患特应性疾病的风险。
比较患湿疹和未患湿疹的幼儿的粪便细菌群落。
巢式病例对照研究。纳入3至6个月大患湿疹的婴儿（病例组，n = 37）和未患湿疹的婴儿（对照组，n = 24），根据性别、年龄、喂养方式（母乳喂养/奶瓶喂养/混合喂养/固体食物喂养）、种族进行匹配。收集有关母婴抗生素暴露、喂养方式、胃肠道症状、过敏家族史的信息。采用特应性皮炎严重程度评分指数进行湿疹严重程度评分。采集样本用于测定过敏原特异性血清IgE（病例组）以及尿液/粪便嗜酸性粒细胞蛋白X。测量胃肠道通透性。分析粪便细菌群落的组成（基于核酸的非培养分析）。
病例组和对照组的粪便细菌群落总体特征没有差异。过敏家族史增加了检测到双歧杆菌的可能性（有家族史，86%；无家族史，56%；P = 0.047）；母乳喂养的婴儿更有可能携带双歧双歧杆菌（比值比，5.19；95%置信区间，1.47 - 18.36；P = 0.01）。在非母乳喂养儿童（比值比，5.6；95%置信区间，1.3 - 24.3；P = 0.02）和患湿疹儿童（湿疹组，26%；无湿疹组，4%；P = 0.04）的粪便中更常检测到假链状双歧杆菌。临床测量指标与假链状双歧杆菌的检测之间没有显著关联。
粪便中假链状双歧杆菌的存在与湿疹及纯配方奶喂养有关；双歧双歧杆菌与母乳喂养有关。
