用于鉴定临床和环境样本中立克次氏体物种的分子方法。
Molecular method for identification of Rickettsia species in clinical and environmental samples.
作者信息
Jado Isabel, Escudero Raquel, Gil Horacio, Jiménez-Alonso María Isabel, Sousa Rita, García-Pérez Ana L, Rodríguez-Vargas Manuela, Lobo Bruno, Anda Pedro
机构信息
Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain.
出版信息
J Clin Microbiol. 2006 Dec;44(12):4572-6. doi: 10.1128/JCM.01227-06. Epub 2006 Oct 11.
Abstract
We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.
摘要
我们提出了一种针对23S-5S内转录间隔区的聚合酶链反应（PCR）方法，并结合反向线印迹技术，可在一步中实现立克次体物种的检测和鉴定。该方法在从患者和环境样本中鉴定立克次体物种方面具有高度的敏感性和特异性。所采用的通用方法使我们能够鉴定出新的病原体。
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