García-Esteban Coral, Gil Horacio, Rodríguez-Vargas Manuela, Gerrikagoitia Xeider, Barandika Jesse, Escudero Raquel, Jado Isabel, García-Amil Cristina, Barral Marta, García-Pérez Ana L, Bhide Mangesh, Anda Pedro
Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain.
J Clin Microbiol. 2008 Feb;46(2):776-9. doi: 10.1128/JCM.01720-07. Epub 2007 Dec 19.
A new, efficient molecular method for detection of Bartonella, based on the 16S-23S rRNA intergenic spacer and 16S rRNA amplification by multiplex PCR combined with reverse line blotting, was designed. This assay could simultaneously detect 20 different known species and other Bartonella species not described previously.
设计了一种基于16S - 23S rRNA基因间隔区和通过多重PCR结合反向线杂交扩增16S rRNA来检测巴尔通体的新型高效分子方法。该检测方法可同时检测20种不同的已知物种以及其他先前未描述的巴尔通体物种。
