Tsubata Y, Sakatsume M, Ogawa A, Alchi B, Kaneko Y, Kuroda T, Kawachi H, Narita I, Yamamoto T, Gejyo F
Department of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Kidney Int. 2006 Dec;70(11):1948-54. doi: 10.1038/sj.ki.5001941. Epub 2006 Oct 11.
Our comprehensive gene expression profiles of the kidneys in an anti-glomerular basement membrane (GBM) nephritis model using DNA arrays revealed that allograft inflammatory factor-1 (AIF-1) was one of the highly expressed genes. Here, we explored the pathological significance of AIF-1 expression in the kidneys. The expression pattern of AIF-1 mRNA and protein in the kidneys of normal and diseased rats, such as anti-GBM nephritis and puromycin aminonucleoside nephrosis, was investigated by in situ hybridization, immunohistochemistry, and immunoelectron microscopy. Furthermore, the expression of AIF-1 in human kidneys and urinary sediments was examined. AIF-1 was expressed at both mRNA and protein levels in podocytes of normal and diseased rats, and in infiltrating cells in anti-GBM nephritis kidneys. The expression of AIF-1 in podocytes was constitutive; positive in podocytes of both normal and diseased rats. In humans, AIF-1 was expressed in podocytes and infiltrating inflammatory cells, similarly. Moreover, it was detected in urinary podocytes from patients with immunoglobulin A nephropathy. These data document for the first time that AIF-1, a constitutively expressed protein in rat and human podocytes, is a novel molecular component of podocytes, and that the upregulation of AIF-1 in an anti-GBM nephritis model may mainly be a consequence of its expression in infiltrating cells.
我们利用DNA阵列对抗肾小球基底膜（GBM）肾炎模型中的肾脏进行全面基因表达谱分析，结果显示同种异体移植炎症因子-1（AIF-1）是高表达基因之一。在此，我们探讨了AIF-1在肾脏中表达的病理意义。通过原位杂交、免疫组织化学和免疫电子显微镜技术，研究了正常和患病大鼠（如抗GBM肾炎和嘌呤霉素氨基核苷肾病大鼠）肾脏中AIF-1 mRNA和蛋白的表达模式。此外，还检测了AIF-1在人肾脏和尿沉渣中的表达。AIF-1在正常和患病大鼠的足细胞以及抗GBM肾炎肾脏中的浸润细胞中均有mRNA和蛋白水平的表达。AIF-1在足细胞中的表达是组成性的；在正常和患病大鼠的足细胞中均呈阳性。在人类中，AIF-1同样在足细胞和浸润性炎症细胞中表达。此外，在免疫球蛋白A肾病患者的尿足细胞中也检测到了AIF-1。这些数据首次证明，AIF-1作为大鼠和人类足细胞中组成性表达的蛋白，是足细胞的一种新型分子成分，并且在抗GBM肾炎模型中AIF-1的上调可能主要是其在浸润细胞中表达的结果。
