Yew N, Oskarsson M, Daar I, Blair D G, Vande Woude G F
ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702.
Mol Cell Biol. 1991 Feb;11(2):604-10. doi: 10.1128/mcb.11.2.604-610.1991.
The mos proto-oncogenes from different vertebrate species transform mouse NIH 3T3 cells with markedly different efficiencies. v-mos, mouse (c-mosmu), and chicken (c-mosch) mos transform NIH 3T3 cells 10- to 100-fold more efficiently than do human (c-moshu) and Xenopus (c-mosxc) mos. The mos genes with the highest transforming activity efficiently induce maturation in Xenopus oocytes and mimic cytostatic factor (CSF) by causing mitotic cleavage arrest in embryos. Chimeric v-mos/c-moshu proteins that had high transforming efficiencies in NIH 3T3 cells were also effective in the induction of oocyte maturation and CSF cleavage arrest. We measured the in vitro autophosphorylation activities of the different mos proteins and found that the levels of kinase activity of v-mos, c-mosmu, and c-mosch were much higher than that of c-mosxc. These data indicate that mos gene transforming efficiency and the ability to induce oocyte maturation or mimic CSF activity are correlated with in vitro autophosphorylation activity and suggest that the mos protein plays a similar role in transformed cells and normal oocytes.
来自不同脊椎动物物种的mos原癌基因以明显不同的效率转化小鼠NIH 3T3细胞。v-mos、小鼠（c-mosmu）和鸡（c-mosch）的mos转化NIH 3T3细胞的效率比人类（c-moshu）和非洲爪蟾（c-mosxc）的mos高10到100倍。具有最高转化活性的mos基因能有效诱导非洲爪蟾卵母细胞成熟，并通过使胚胎中的有丝分裂分裂停滞来模拟细胞静止因子（CSF）。在NIH 3T3细胞中具有高转化效率的嵌合v-mos/c-moshu蛋白在诱导卵母细胞成熟和CSF分裂停滞方面也很有效。我们测量了不同mos蛋白的体外自磷酸化活性，发现v-mos、c-mosmu和c-mosch的激酶活性水平远高于c-mosxc。这些数据表明，mos基因的转化效率以及诱导卵母细胞成熟或模拟CSF活性的能力与体外自磷酸化活性相关，并表明mos蛋白在转化细胞和正常卵母细胞中发挥相似作用。
