Voelker R A, Gibson W, Graves J P, Sterling J F, Eisenberg M T
Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Mol Cell Biol. 1991 Feb;11(2):894-905. doi: 10.1128/mcb.11.2.894-905.1991.
The nucleotide sequence of the Drosophila melanogaster suppressor of sable [su(s)] gene has been determined. Comparison of genomic and cDNA sequences indicates that an approximately 7,860-nucleotide primary transcript is processed into an approximately 5-kb message, expressed during all stages of the life cycle, that contains an open reading frame capable of encoding a 1,322-amino-acid protein of approximately 150 kDa. The putative protein contains an RNA recognition motif-like region and a highly charged arginine-, lysine-, serine-, aspartic or glutamic acid-rich region that is similar to a region contained in several RNA-processing proteins. In vitro translation of in vitro-transcribed RNA from a complete cDNA yields a product whose size agrees with the size predicted by the open reading frame. Antisera against su(s) fusion proteins recognize the in vitro-translated protein and detect a protein of identical size in the nuclear fractions from tissue culture cells and embryos. The protein is also present in smaller amounts in cytoplasmic fractions of embryos. That the su(s) protein has regions similar in structure to RNA-processing protein is consistent with its known role in affecting the transcript levels of those alleles that it suppresses.
已确定黑腹果蝇黑貂抑制因子[su(s)]基因的核苷酸序列。基因组序列与cDNA序列的比较表明，一个约7860个核苷酸的初级转录本被加工成一个约5kb的信使RNA，在生命周期的所有阶段均有表达，该信使RNA包含一个开放阅读框，能够编码一个约150kDa的1322个氨基酸的蛋白质。推测的蛋白质含有一个类似RNA识别基序的区域和一个高度带电的富含精氨酸、赖氨酸、丝氨酸、天冬氨酸或谷氨酸的区域，该区域与几种RNA加工蛋白中的一个区域相似。从完整的cDNA体外转录的RNA进行体外翻译，产生的产物大小与开放阅读框预测的大小一致。针对su(s)融合蛋白的抗血清识别体外翻译的蛋白，并在来自组织培养细胞和胚胎的核部分中检测到大小相同的蛋白。该蛋白在胚胎的细胞质部分中也有少量存在。su(s)蛋白具有与RNA加工蛋白结构相似的区域，这与其在影响其抑制的那些等位基因的转录水平方面的已知作用是一致的。
