通过琼脂糖凝胶电泳分析缺陷型SV40 DNA。

Analysis of defective SV40 DNA by agarose gel electrophoresis.

作者信息

Sol C J, Walig C, ter Schegget J, van der Noordaa J

出版信息

J Gen Virol. 1975 Sep;28(3):285-97. doi: 10.1099/0022-1317-28-3-285.

Abstract

The SV40 DNA that was generated by undiluted passaging of the virus was analysed by agarose gel electrophoresis. Nine bands of virus DNA were distinguished and each band contained a specific size class of DNA, all shorter than the complete SV40 genome as was determined by electron microscopy measurements. A difference of 2% in length, about 100 base pairs, resulted in a clear band splitting. Two sets of undiluted passaging were established and the defective DNA in the two sets had both different and similar size classes varying in length from 96% to 73% of the unit length SV40 DNA.

摘要

对通过病毒原液传代产生的SV40 DNA进行琼脂糖凝胶电泳分析。区分出九条病毒DNA条带，每条带都包含特定大小类别的DNA，所有这些DNA都比通过电子显微镜测量确定的完整SV40基因组短。长度相差2%，约100个碱基对，会导致条带明显分裂。建立了两组原液传代，两组中的缺陷DNA具有不同和相似的大小类别，长度从SV40单位长度DNA的96%到73%不等。