Kotlikoff Michael I
Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401, USA.
J Physiol. 2007 Jan 1;578(Pt 1):55-67. doi: 10.1113/jphysiol.2006.120212. Epub 2006 Oct 12.
This article reviews genetically encoded Ca2+ indicators (GECIs), with a focus on the use of these novel molecules in the context of understanding complex cell signalling in mammals, in vivo. The review focuses on the advantages and limitations of specific GECI design strategies and the results of experiments in which these molecules have been expressed in transgenic mice, concentrating particularly on recent experiments from our laboratory in which physiological signalling could be monitored in vivo. Finally, newer strategies for effective genetic specification of GECIs are briefly reviewed.
本文综述了基因编码的钙离子指示剂（GECIs），重点关注这些新型分子在理解哺乳动物体内复杂细胞信号传导方面的应用。综述聚焦于特定GECI设计策略的优缺点，以及这些分子在转基因小鼠中表达的实验结果，特别关注我们实验室最近的实验，其中可以在体内监测生理信号传导。最后，简要综述了有效遗传指定GECIs的新策略。
