多位点酶电泳支持喀麦隆疟蚊尼里组内的物种形成。

Multilocus enzyme electrophoresis supports speciation within the Anopheles nili group of malaria vectors in Cameroon.

作者信息

Awono-Ambene Herman P, Simard Frédéric, Antonio-Nkondjio Christophe, Cohuet Anna, Kengne Pierre, Fontenille Didier

机构信息

Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Yaoundé, Cameroon.

出版信息

Am J Trop Med Hyg. 2006 Oct;75(4):656-8.

PMID:17038689

Abstract

Multilocus enzyme analysis of the genetic variability and population structure was conducted among three malaria vector species of the Anopheles nili group in Cameroon: An. nili, An. carnevalei, and the recently described An. ovengensis. We detected species-specific alleles and large differences in shared allele frequencies at six of nine loci (e.g., PGM, GOT1, IDH1, IDH2, PGI, and alpha-GPD). This non-random distribution of alleles leads to high and significant values of differentiation indexes (0.569 < Fst < 0.874, P < 10(-4)). These results fully agree with standard morphologic descriptions, and therefore provide further support for recent taxonomic classification within the An. nili group.

摘要

对喀麦隆尼罗按蚊组的三种疟疾媒介物种进行了多位点酶分析，以研究其遗传变异性和种群结构，这三种物种分别是尼罗按蚊、卡氏按蚊和最近描述的奥温按蚊。我们在九个位点中的六个位点（例如，磷酸葡萄糖变位酶（PGM）、谷草转氨酶1（GOT1）、异柠檬酸脱氢酶1（IDH1）、异柠檬酸脱氢酶2（IDH2）、磷酸葡萄糖异构酶（PGI）和α-甘油磷酸脱氢酶（alpha-GPD））检测到了物种特异性等位基因以及共享等位基因频率的巨大差异。这种等位基因的非随机分布导致分化指数的值很高且具有显著性（0.569 < Fst < 0.874，P < 10(-4)）。这些结果与标准形态学描述完全一致，因此为尼罗按蚊组内最近的分类学分类提供了进一步支持。