[哈尔明诱导人SGC-7901细胞凋亡]
[Harmine induces apoptosis in human SGC-7901 cells].
作者信息
Song Zhen-ya, Liu Ji-ren, Lu Xin-liang, Wang Liang-jing
机构信息
Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.
出版信息
Zhong Yao Cai. 2006 Jun;29(6):571-3.
OBJECTIVE
[corrected] To investigate the effect of apoptosis induced in human SGC-7901 cells by Harmine.
METHODS
The effect of Harmine on human SGC-7901 cell survival and apoptosis was determined by MTT assay, light microscopy and flow cytometry. Cell genomic DNA was detected by agarose electrophoresis.
RESULTS
The survival of human SGC-7901 cells decreased; Apoptotic cells were observed by fluorescent microscope; FCM analysis showed that the peak of apoptosis increased. Typical DNA Ladder were detected in DNA agarose gel electrophoresis.
CONCLUSION
HM can induce apoptosis in human SGC-7901 cells.
目的
研究去氢骆驼蓬碱诱导人SGC - 7901细胞凋亡的作用。
方法
采用MTT法、光学显微镜及流式细胞术检测去氢骆驼蓬碱对人SGC - 7901细胞存活及凋亡的影响。通过琼脂糖电泳检测细胞基因组DNA。
结果
人SGC - 7901细胞存活率下降；荧光显微镜下观察到凋亡细胞；流式细胞术分析显示凋亡峰升高。DNA琼脂糖凝胶电泳检测到典型的DNA梯状条带。
结论
去氢骆驼蓬碱可诱导人SGC - 7901细胞凋亡。
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