Vega-Maray Ana María, Fernández-González Delia, Valencia-Barrera Rosa, Suárez-Cervera María
Department of Vegetal Biology (Botany), Faculty of Biology, University of León, Spain.
Ann Allergy Asthma Immunol. 2006 Sep;97(3):343-9. doi: 10.1016/S1081-1206(10)60799-5.
Allergy to the pollen of flowering plant species significantly affects the health of people in many parts of the world. Pollens of related genera usually share common antigens and are often, but not always, cross-reactive. Several studies have shown that Parietaria pollen is one of the most common causes of pollinosis in the Mediterranean area, whereas Urtica has no allergenic significance.
To report on the localization of Parietaria judaica major allergen in Urtica dioica pollen grains and on the detection of allergenic proteins in U. dioica pollen grains during the hydration-activation process.
A combination of transmission electron microscopy and immunocytochemical methods was used to locate allergenic proteins in U. dioica pollen grains after different periods of hydration-activation using the anti-Par j 1 (4.1.3.) monoclonal antibody and serum samples from allergic patients.
No significant labeling was noted for Parj 1 allergen after 10, 15, and 20 minutes in the walls and cytoplasm. Slight labeling was observed for allergic proteins in the walls of U. dioica after 10 minutes of hydration, and no significant labeling was found after 15 and 20 minutes of hydration.
Immunocytochemical methods confirmed the absence of cross-reactivity between 2 related genera, Parietaria and Urtica, and the lowest allergenic potential of U. dioica.
对开花植物花粉的过敏严重影响着世界许多地区人们的健康。相关属的花粉通常具有共同抗原，且常常（但并非总是）存在交叉反应。多项研究表明，墙草花粉是地中海地区花粉症最常见的病因之一，而荨麻花粉并无致敏意义。
报告药用墙草主要过敏原在异株荨麻花粉粒中的定位情况，以及在水合激活过程中异株荨麻花粉粒中致敏蛋白的检测结果。
采用透射电子显微镜和免疫细胞化学方法相结合，使用抗墙草属第1号过敏原（4.1.3.）单克隆抗体和过敏患者血清样本，对水合激活不同时间段后的异株荨麻花粉粒中的致敏蛋白进行定位。
在10分钟、15分钟和20分钟时，细胞壁和细胞质中均未发现墙草属第1号过敏原的显著标记。水合10分钟后，在异株荨麻细胞壁中观察到过敏蛋白的轻微标记，水合15分钟和20分钟后未发现显著标记。
免疫细胞化学方法证实了墙草属和荨麻属这两个相关属之间不存在交叉反应，且异株荨麻的致敏潜力最低。
