Casas C, Márquez J, Suárez-Cervera M, Seoane-Camba J A
Department of Botany, Faculty of Pharmacy, University of Barcelona, Spain.
Eur J Cell Biol. 1996 Jun;70(2):179-88.
Inhalation of pollen grains and fungal spores leads to allergenic rhinitis and type I asthma mediated by IgE. The etiology of these conditions is due to the presence of substances that can induce anaphylactic processes in susceptible subjects. A combination of transmission electron microscopy with immunocytochemical methods was used to localize allergenic proteins in Parietaria judaica L. (Urticaceae) pollen grains. Germination of pollen grains was induced in vitro for 10 and 30 min to activate proteins in the intine and in material exuded from the pollen grains that are related to pollen-stigma recognition; these activated proteins would be the ones that react with the antibodies. The activation time, labeling intensity and specificity of the antigens depended on whether IgG (rabbit) or IgE (allergenic patient) was used. Abundant, non-specific labeling was observed with rabbit IgG. The labeling observed with human IgE was located more specifically in the intine (oncus) at random points near the apertures. The immunolocalization of allergenic proteins in the intine indicates the presence of proteins related to activation of the pollen grains. This fact confirms the function attributed to the intine in the processes of pollen tube formation and fertilization, and also the possible mechanism that is activated in the pollen grains when these reach the mucosa of sensitive subjects. In the case of P. judaica, the existence of a thickening of the intine below the apertures (oncus) and the release of its contents in an explosive fashion seem to be the main causes of their strong allergenic activity.
吸入花粉颗粒和真菌孢子会导致由IgE介导的变应性鼻炎和I型哮喘。这些病症的病因是存在能够在易感个体中诱发过敏反应过程的物质。采用透射电子显微镜与免疫细胞化学方法相结合的技术，对墙草属花粉颗粒中的变应原蛋白进行定位。在体外诱导花粉颗粒萌发10分钟和30分钟，以激活花粉内壁和从花粉颗粒中渗出的与花粉-柱头识别相关物质中的蛋白质；这些被激活的蛋白质就是会与抗体发生反应的蛋白质。抗原的激活时间、标记强度和特异性取决于使用的是IgG（兔源）还是IgE（变应性患者源）。用兔IgG观察到大量非特异性标记。用人IgE观察到的标记更特异性地位于孔口附近随机点处的内壁（瘤状加厚处）。变应原蛋白在内壁中的免疫定位表明存在与花粉颗粒激活相关的蛋白质。这一事实证实了内壁在花粉管形成和受精过程中所具有的功能，也证实了花粉颗粒到达敏感个体黏膜时可能被激活的机制。就墙草属而言，孔口下方内壁的加厚（瘤状加厚处）及其内容物的爆发式释放似乎是其强烈变应原活性的主要原因。
