Chen Sheng-Xia, Yang Xiao-Ming, Wu Liang, Li Long-Gen, Zhang Rong-Xian, Xia Lei, Shao Shi-He
School of Medical Technology, Jiangsu University, Zhenjiang 212013, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Feb 28;25(1):45-8.
To observe the molluscicidal activity and the fish acute toxicity of the molluscicides extracted from Ginkgo biloba sarcotesta by benzinum (EGSB) (with major component of ginkgolic acid), arecoline (ARE) and their combination.
Oncomelania hupensis snails were immersed in different concentrations of dry powder sarcotesta of Ginkgo biloba (DPGB), extract of Ginkgo biloba sarcotesta by water (EGSW) and EGSB by WHO recommended method for molluscicide screening to observe the molluscicidal activity, and also the inhibiting effect on the snails' climbing-up as well as acute toxicity to Brachydanio rerio. Niclosamide was used as control.
The three extractions from Ginkgo biloba all showed molluscicidal activity, with EGSB as the best. Its 24 h LC50 and LC90 were 0.65 mg/L and 5.50 mg/L, and the 48 h LC50 and LC90 were 0.07 mg/L and 0.85 mg/L, respectively. The combination of EGSB and ARE showed better effect than EGSB alone. Its 24 h LC50 and LC90 were 0.26 mg/L and 0.56 mg/L respectively, a sharp decrease by 60% and 90% compared to EGSB (P<0.05). Under the concentration of 2.50 mg/L of EGSB, the rate of snails' climbing-up was 10%, while under the concentration of 0.16 mg/L of the EGSB+ARE combination, the rate was 8%. The inhibition on the snails' climbing-up of the combination was stronger than EGSB. The fish survived for 24 h and 10th respectively at the concentration of 1 x LC90 and 2 x LC90 of EGSB. Under the concentration of 2 x LC90 of the combination, only 50% of the fish died and no fish died at the concentration of 1 x LC90. The toxicity of the combination was lower than EGSB alone.
EGSB shows an adequate molluscicidal activity and it is worth of further investigation.
观察用苯提取的银杏外种皮提取物（EGSB，主要成分为银杏酸）、槟榔碱（ARE）及其组合的杀螺活性和鱼类急性毒性。
采用世界卫生组织推荐的杀螺剂筛选方法，将钉螺浸泡在不同浓度的银杏外种皮干粉（DPGB）、银杏外种皮水提取物（EGSW）和EGSB中，观察其杀螺活性，以及对钉螺上爬的抑制作用和对斑马鱼的急性毒性。以氯硝柳胺作为对照。
银杏的三种提取物均表现出杀螺活性，其中EGSB效果最佳。其24 h LC50和LC90分别为0.65 mg/L和5.50 mg/L，48 h LC50和LC90分别为0.07 mg/L和0.85 mg/L。EGSB与ARE的组合效果优于单独的EGSB。其24 h LC50和LC90分别为0.26 mg/L和0.56 mg/L，与EGSB相比分别大幅下降了60%和90%（P<0.05）。在2.50 mg/L的EGSB浓度下，钉螺上爬率为10%，而在0.16 mg/L的EGSB+ARE组合浓度下，上爬率为8%。该组合对钉螺上爬的抑制作用强于EGSB。在EGSB的1×LC90和2×LC90浓度下，鱼类分别存活24 h和10天。在该组合的2×LC90浓度下，只有50%的鱼死亡，在1×LC90浓度下无鱼死亡。该组合的毒性低于单独的EGSB。
EGSB表现出足够的杀螺活性，值得进一步研究。
