Fang Wei-Wei, Chen Jun, Han Bang-Xing, Li Chen-Guang, Yang Xue-Juan, Zhang Chun-Xiao, Yao Hu
School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2011 Aug;23(4):449-52.
To study the molluscicidal activity, the influence on glycogen content of Oncomelania hupensis and the acute toxicity to zebra fish of the extract from Phytolacca americana Linn leaf.
The different polar factions of the extract of Phytolacca americana Linn leaf were separated by using the systemic solvent segregation method, and then the molluscicidal activity of the fractions was detected according to the Laboratory Final Milluscicides Screening Method issued by WHO. The glycogen content of soft tissues of Oncomelania hupensis treated by the ethyl acetate polar fraction was determined by the anthrone method. Finally, the acute toxicity of the ethyl acetate polar fraction to non-targets was studied with zebra fish.
The ethyl acetate polar fraction was the best active components against the snails. Its 48 h LC50 and LC90 were 6.0 mg/100 ml and 26.1 mg/ 100 ml, respectively. The glycogen content of soft tissues of the snails decreased by 20% after treated with the fraction. The fish treated by the concentration of LC50 (48 h) of the ethyl acetate polar fraction survived for 12 h.
The Phytolacca americana Linn leaf possesses an adequate molluscicidal activity and a significant acute toxicity to the zebra fish.
研究美洲商陆叶提取物的杀螺活性、对钉螺糖原含量的影响以及对斑马鱼的急性毒性。
采用系统溶剂分离法分离美洲商陆叶提取物的不同极性部位，然后按照世界卫生组织发布的《实验室终末杀螺剂筛选方法》检测各部位的杀螺活性。采用蒽酮法测定经乙酸乙酯极性部位处理的钉螺软组织中的糖原含量。最后，用斑马鱼研究乙酸乙酯极性部位对非靶标的急性毒性。
乙酸乙酯极性部位是对钉螺活性最佳的成分。其48 h半数致死浓度（LC50）和90%致死浓度（LC90）分别为6.0 mg/100 ml和26.1 mg/100 ml。用该部位处理后，钉螺软组织中的糖原含量下降了20%。经乙酸乙酯极性部位48 h LC50浓度处理的鱼存活了12 h。
美洲商陆叶具有足够的杀螺活性，对斑马鱼有显著的急性毒性。
