Giannubilo Stefano R, Menegazzi Marta, Tedeschi Elisa, Bezzeccheri Valeria, Suzuki Hisanori, Tranquilli Andrea L
Department of Obstetrics and Gynecology, Polytechnic University of Marche, Ancona, Italy.
J Matern Fetal Neonatal Med. 2008 Sep;21(9):617-22. doi: 10.1080/14767050802214774.
To assess placental nitric oxide (NO) metabolism related to changes in the uteroplacental circulation during fetal growth restriction (FGR).
The resistance index (RI) from the uterine arteries and pulsatility index (PI) from the umbilical artery were determined by Doppler analysis in 15 patients with FGR and 12 healthy controls, before elective cesarean section. Inducible (iNOS) and endothelial (eNOS) NO synthase expression were measured in placental samples. Immunohistochemistry was performed for iNOS location in the placenta.
During FGR, we observed a significant elevation of iNOS when compared with controls. Conversely, eNOS did not differ between the two groups. A negative correlation with eNOS (r = -0.85) and a positive correlation with iNOS (r = 0.91) was found correlating to umbilical PI. The iNOS proteins were reduced in syncytiotrophoblast cells and increased in endothelium in the FGR group compared to the controls.
During FGR, placental iNOS expression is significantly increased; this increase possibly represents an adaptive physiological mechanism for overcoming a fetoplacental circulation deficiency.
评估胎儿生长受限（FGR）期间与子宫胎盘循环变化相关的胎盘一氧化氮（NO）代谢。
在择期剖宫产术前，通过多普勒分析测定了15例FGR患者和12例健康对照者子宫动脉的阻力指数（RI）和脐动脉的搏动指数（PI）。检测胎盘样本中诱导型（iNOS）和内皮型（eNOS）一氧化氮合酶的表达。对胎盘进行iNOS定位的免疫组织化学检测。
与对照组相比，FGR期间我们观察到iNOS显著升高。相反，两组之间eNOS无差异。发现脐动脉PI与eNOS呈负相关（r = -0.85），与iNOS呈正相关（r = 0.91）。与对照组相比，FGR组合体滋养层细胞中的iNOS蛋白减少，而内皮细胞中的iNOS蛋白增加。
在FGR期间，胎盘iNOS表达显著增加；这种增加可能代表一种克服胎儿胎盘循环不足的适应性生理机制。
