Vitoratos Nicolaos, Papadias Constantinos, Economou Emmanuel, Makrakis Evangelos, Panoulis Constantinos, Creatsas George
2nd Department of Obstetrics and Gynecology, Medical School, University of Athens, Aretaieion Hospital, Athens, Greece.
Mediators Inflamm. 2006;2006(4):30485. doi: 10.1155/MI/2006/30485.
The purpose of the present study was to examine the profile of selected proinflammatory cytokines in maternal serum of first-trimester pregnancies complicated by threatened abortion (TACP) and its relevance to obstetric outcome. Serum levels of Th1-type cytokines interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), and Th2-type cytokine interleukin 6 (IL-6) were measured, by ELISA, in 22 women with TACP and adverse outcome at admission (group A) and compared with the corresponding levels of 31 gestational age-matched women with TACP and successful outcome at admission (group B1) and discharge (group B2) and 22 gestational age-matched women with first-trimester uncomplicated pregnancy (group C) who served as controls. Mann-Whitney U or Wilcoxon test was applied as appropriate to compare differences between groups. IL-1beta and TNF-alpha were detected with significantly higher levels in group A, compared to all other groups. On the contrary, IL-6 levels were detected with no significant difference among all the other groups studied. It is concluded that in first-trimester TACP with adverse outcome, a distinct immune response, as reflected by elevated maternal IL-1beta, TNF-alpha, and unaltered IL-6 levels, is relevant to a negative obstetric outcome.
本研究的目的是检测孕早期并发先兆流产（TACP）孕妇血清中某些促炎细胞因子的概况及其与产科结局的相关性。通过酶联免疫吸附测定法（ELISA）测量了22例入院时患有TACP且结局不良的孕妇（A组）血清中Th1型细胞因子白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）以及Th2型细胞因子白细胞介素-6（IL-6）的水平，并与31例入院时患有TACP且结局良好（B1组）及出院时结局良好（B2组）的孕龄匹配孕妇以及22例孕龄匹配的孕早期无并发症孕妇（C组，作为对照组）的相应水平进行比较。根据情况应用曼-惠特尼U检验或威尔科克森检验来比较组间差异。与所有其他组相比，A组中检测到的IL-1β和TNF-α水平显著更高。相反，在所研究的所有其他组中，IL-6水平检测无显著差异。结论是，在结局不良的孕早期TACP中，母体IL-1β、TNF-α升高以及IL-6水平未改变所反映的独特免疫反应与不良产科结局相关。
