Paster B J, Lee A, Fox J G, Dewhirst F E, Tordoff L A, Fraser G J, O'Rourke J L, Taylor N S, Ferrero R
Forsyth Dental Center, Boston, Massachusetts 02115.
Int J Syst Bacteriol. 1991 Jan;41(1):31-8. doi: 10.1099/00207713-41-1-31.
Strain CS1T (T = type strain) is a gram-negative, microaerophilic, urease-positive, spiral-shaped bacterium that was isolated from the gastric mucosa of a cat. Additional strains which possessed biochemical and ultrastructural characteristics similar to those of strain CS1T were isolated from the gastric mucosa of cats and dogs. The guanine-plus-cytosine content of the DNA of strain CS1T was 42.5 mol%. The 16S rRNA sequences of strain CS1T, strain DS3 (a spiral-shaped isolate from a dog), and Helicobacter mustelae were determined by direct RNA sequencing, using a modified Sanger method. These sequences were compared with the 16S rRNA sequences of Helicobacter pylori, "Flexispira rappini," Wolinella succinogenes, and 11 species of campylobacters. A dendrogram was constructed based upon sequence similarities. Strains CS1T and DS3 were very closely related (level of similarity, 99.3%). Two major phylogenetic groups were formed; one group consisted of strains CS1T and DS3, H. mustelae, H. pylori, "F. rappini," and W. succinogenes, and the other group contained the true campylobacters. The average level of similarity between members of these two groups was 84.9%. Within the first group, strains CS1T and DS3, H. pylori, and H. mustelae formed a cluster of organisms with an interspecies similarity level of 94.5%. The phylogenetic positions of W. succinogenes and "F. rappini" were just outside this cluster. On the basis of the results of this study, we believe that strains CS1T (= ATCC 49179T) and DS3 represent a new species of the genus Helicobacter, for which we propose the name Helicobacter felis.
CS1T菌株（T = 模式菌株）是一种革兰氏阴性、微需氧、脲酶阳性的螺旋形细菌，从一只猫的胃黏膜中分离得到。从猫和狗的胃黏膜中还分离出了其他一些菌株，它们具有与CS1T菌株相似的生化和超微结构特征。CS1T菌株DNA的鸟嘌呤加胞嘧啶含量为42.5摩尔%。采用改良的桑格法，通过直接RNA测序确定了CS1T菌株、DS3菌株（从一只狗身上分离出的螺旋形菌株）和幽门螺杆菌的16S rRNA序列。将这些序列与幽门螺杆菌、“拉平弯曲螺杆菌”、产琥珀酸沃林氏菌以及11种弯曲杆菌的16S rRNA序列进行了比较。根据序列相似性构建了系统发育树状图。CS1T菌株和DS3菌株密切相关（相似性水平为99.3%）。形成了两个主要的系统发育组；一组由CS1T菌株、DS3菌株、幽门螺杆菌、“拉平弯曲螺杆菌”和产琥珀酸沃林氏菌组成，另一组包含真正的弯曲杆菌。这两组成员之间的平均相似性水平为84.9%。在第一组中，CS1T菌株、DS3菌株、幽门螺杆菌和幽门螺杆菌形成了一个种间相似性水平为94.5%的生物体簇。产琥珀酸沃林氏菌和“拉平弯曲螺杆菌”的系统发育位置就在这个簇之外。根据这项研究的结果，我们认为CS1T菌株（= ATCC 49179T）和DS3菌株代表了幽门螺杆菌属的一个新物种，我们为此提议将其命名为猫幽门螺杆菌。
