Bronsdon M A, Goodwin C S, Sly L I, Chilvers T, Schoenknecht F D
Regional Primate Research Center, University of Washington, Seattle 98195.
Int J Syst Bacteriol. 1991 Jan;41(1):148-53. doi: 10.1099/00207713-41-1-148.
A new microaerophilic, spirally curved, rod-shaped bacterium was isolated from the gastric mucosa of a pigtailed macaque (Macaca nemestrina). The gram-negative cells of this bacterium are oxidase, catalase, and urease positive and strongly resemble Helicobacter pylori (Campylobacter pylori) cells. Like H. pylori, this organism does not metabolize glucose, does not reduce nitrate or produce indole, does not produce H2S from triple sugar iron agar, does not hydrolyze hippurate or esculin, and does not grow in the presence of 1% glycine, 1.5% salt, or 1% bile. Also like H. pylori, it is resistant to nalidixic acid and susceptible to cephalothin. However, unlike H. pylori, the colorless colonies are flat and have irregular edges. This organism has a unique cellular fatty acid composition, forming a new gas-liquid chromatography group, group K, and a distinctive DNA content (24 mol% guanine plus cytosine). It exhibits less than 10% DNA-DNA homology (as determined by the nylon filter blot method at 65 degrees C) with other members of the genus Helicobacter. Although the levels of DNA relatedness between previously described Helicobacter species and the new organism are low (less than 10%) and the difference in guanine-plus-cytosine content is large (24 versus 36 to 41 mol%), the genus Helicobacter is the only genus in which it is logical to include the organism at this time. We propose that our single strain represents a new species, Helicobacter nemestrinae, and we designate strain T81213-NTB (= ATCC 49396) as the type strain.
从豚尾猕猴（食蟹猴）的胃黏膜中分离出一种新的微需氧、螺旋状弯曲的杆状细菌。该细菌的革兰氏阴性菌细胞氧化酶、过氧化氢酶和脲酶呈阳性，与幽门螺杆菌（幽门弯曲菌）细胞极为相似。与幽门螺杆菌一样，这种微生物不代谢葡萄糖，不还原硝酸盐或产生吲哚，不在三糖铁琼脂上产生硫化氢，不水解马尿酸盐或七叶苷，并且在1%甘氨酸、1.5%盐或1%胆汁存在的情况下不生长。同样与幽门螺杆菌一样，它对萘啶酸耐药，对头孢噻吩敏感。然而，与幽门螺杆菌不同的是，其无色菌落扁平且边缘不规则。这种微生物具有独特的细胞脂肪酸组成，形成了一个新的气-液色谱组，即K组，以及独特的DNA含量（鸟嘌呤加胞嘧啶为24摩尔%）。与幽门螺杆菌属的其他成员相比，它表现出低于10%的DNA-DNA同源性（通过65℃尼龙滤膜印迹法测定）。尽管先前描述的幽门螺杆菌属物种与这种新微生物之间的DNA相关性水平较低（低于10%），且鸟嘌呤加胞嘧啶含量差异较大（24对36至41摩尔%），但幽门螺杆菌属是目前将这种微生物纳入其中合乎逻辑的唯一属。我们提议我们的单一菌株代表一个新物种，即食蟹猴幽门螺杆菌，我们将菌株T81213-NTB（=ATCC 49396）指定为模式菌株。
