Warg Janet V, Dikkeboom Audrey L, Goodwin Andrew E, Snekvik Kevin, Whitney John
Diagnostic Virology Laboratory, National Veterinary Services Laboratories, VS-APHIS-USDA, Ames, IA 50010, USA.
Virus Genes. 2007 Aug;35(1):87-95. doi: 10.1007/s11262-006-0042-3. Epub 2006 Oct 18.
Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.
2002年至2004年期间，在美国分离出了5株鲤春病毒血症病毒（SVCV），即鲤弹状病毒。采用单管逆转录聚合酶链反应（RT-PCR）从美国的SVCV分离株中生成重叠的cDNA片段。设计了多对特异性引物，以扩增SVCV基因组Id的磷蛋白基因、基质基因和糖蛋白基因的一部分（对应于GenBank登录号NC_002803的核苷酸2174 - 4942）。对序列进行校对和比对，以生成每个分离株的共有序列。对2705个核苷酸的共有序列进行系统发育分析表明，所有5株美国分离株均属于SVCV基因组Ia，即亚洲起源的分离株，并鉴定出了SVCV基因组Ia特有的PCR引物结合位点。
