Dixon P F, Longshaw C B
CEFAS Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.
Dis Aquat Organ. 2005 Nov 9;67(1-2):25-9. doi: 10.3354/dao067025.
Two test kits for the identification of spring viraemia of carp virus (SVCV), one an enzyme-linked immunosorbent assay (ELISA) using a rabbit polyclonal antiserum, and the other an indirect fluorescent antibody test (IFAT) using a mouse monoclonal antibody, were assessed for specificity using a range of virus isolates. The test viruses were selected from 4 recently described genogroups of piscine rhabdoviruses: Genogroup I (SVCV), Genogroup II (grass carp rhabdovirus), Genogroup III (pike fry rhabdovirus) and Genogroup IV ('tench rhabdovirus'). The test viruses included SVCV isolates from all 4 subgroups of Genogroup I. The ELISA was non-specific for these viruses and did not distinguish between SVCV and isolates from the other 3 Genogroups. However, the IFAT was too specific and detected SVCV isolates from only 1 of the 4 SVCV subgroups. Reliance on these test kits alone could result in misidentification of this OIE notifiable disease.
使用一系列病毒分离株对两种用于鉴定鲤春病毒血症病毒（SVCV）的检测试剂盒进行了特异性评估，其中一种是使用兔多克隆抗血清的酶联免疫吸附测定（ELISA），另一种是使用小鼠单克隆抗体的间接荧光抗体试验（IFAT）。测试病毒选自最近描述的4个鱼类弹状病毒基因组群：基因组群I（SVCV）、基因组群II（草鱼弹状病毒）、基因组群III（梭鲈幼鱼弹状病毒）和基因组群IV（“丁鱥弹状病毒”）。测试病毒包括基因组群I所有4个亚组的SVCV分离株。ELISA对这些病毒无特异性，无法区分SVCV和其他3个基因组群的分离株。然而，IFAT过于特异，仅能检测到4个SVCV亚组中1个亚组的SVCV分离株。仅依靠这些检测试剂盒可能会导致对这种世界动物卫生组织应通报疾病的错误鉴定。
