Riemann M, Gutjahr C, Korte A, Riemann M, Danger B, Muramatsu T, Bayer U, Waller F, Furuya M, Nick P
Botanisches Institut 1, Technische Universität Karlsruhe, Germany.
Plant Biol (Stuttg). 2007 Jan;9(1):32-40. doi: 10.1055/s-2006-924561. Epub 2006 Oct 17.
The reaction of the rice mutant HEBIBA differs from that of wild-type rice in that the mutant responds inversely to red light and is defective in the light-triggered biosynthesis of jasmonic acid (JA). Using the wild type and the HEBIBA mutant of rice in a differential display screen, we attempted to identify genes that act in or near the convergence point of light and JA signalling. We isolated specifically regulated DNA fragments from approximately 10 000 displayed bands, and identified a new early light- and JA-induced gene. This gene encodes an enzyme containing a GDSL motif, showing 38 % identity at the amino acid level to lipase Arab-1 in Arabidopsis thaliana. The GDSL CONTAINING ENZYME RICE 1 gene (GER1) is rapidly induced by both red (R) and far-red (FR) light and by JA. The results are discussed with respect to a possible role for GER1 as a negative regulator of coleoptile elongation in the context of recent findings on the impact of JA on light signalling.
水稻突变体HEBIBA的反应与野生型水稻不同，该突变体对红光的反应相反，且在光触发的茉莉酸（JA）生物合成方面存在缺陷。我们利用野生型水稻和HEBIBA突变体进行差异显示筛选，试图鉴定在光和JA信号转导交汇点或其附近起作用的基因。我们从大约10000条显示条带中分离出特异性调控的DNA片段，并鉴定出一个新的早期光诱导和JA诱导基因。该基因编码一种含有GDSL基序的酶，在氨基酸水平上与拟南芥中的脂肪酶Arab-1具有38%的同一性。含GDSL基序的酶水稻1基因（GER1）可被红光（R）、远红光（FR）和JA迅速诱导。结合最近关于JA对光信号转导影响的研究结果，对GER1作为胚芽鞘伸长负调控因子的可能作用进行了讨论。
