用于中华夏蜡梅鉴别的种特异性SCAR标记。
Species-specific SCAR markers for authentication of Sinocalycanthus chinensis.
作者信息
Ye Qian, Qiu Ying-xiong, Quo Yan-qi, Chen Jian-xin, Yang Shu-zhen, Zhao Ming-shui, Fu Cheng-xin
机构信息
Laboratory of Systematic and Evolutionary Botany and Biodiversity, School of Life Sciences, Zhejiang University, Hangzhou 310029, China.
出版信息
J Zhejiang Univ Sci B. 2006 Nov;7(11):868-72. doi: 10.1631/jzus.2006.B0868.
Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.
夏蜡梅是中国特有的濒危物种，在中国作为观赏景观树进行栽培。然而，由于夏蜡梅、蜡梅属植物和美国蜡梅的形态特征相似，在苗木市场上很难区分它们。在本研究中，利用简单序列重复区间（ISSR）技术从其近缘物种中检测夏蜡梅。在所有夏蜡梅种质中均发现了一条独特的748 bp条带。通过对该特异性条带进行克隆和测序，并设计一对引物扩增748 bp条带，创建了序列特异性扩增区域（SCAR）标记。以夏蜡梅、蜡梅属植物和美国蜡梅的总DNA为模板，使用该引物对进行诊断性PCR，结果只有夏蜡梅能够被扩增。这种扩增不仅快速（不到3小时即可获得结果），而且易于操作。因此，它是一种在苗木市场上鉴定夏蜡梅的可行方法。
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