[反相高效液相色谱法测定红花中红花黄色素A及资源品质比较]
[Determination of safflor yellow A by RP-HPLC and resources quality comparison in Carthamus tinctorius].
作者信息
Guo Mei-li, Zhang Ge, Zhang Wei, Zhang Han-ming, Su Zhong-wu
机构信息
College of Pharmacy, Second Military Medical University, Shanghai, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Aug;31(15):1234-6.
OBJECTIVE
To provide the basis for establishing evaluation criterion, selecting good strains and carring out good agricultural practice of the crude drug.
METHOD
Representative 22 varieties of Carthamus tinctorius were selected and cultivated in different ecological localities and different years. And the content of safflor yellow A in their corollas were measured by RP-HPLC to compare the differences and their genetic stabilities among varieties.
RESULT
The range of of safflor yellow A content was 0.70%-1.85% which were varied among varieties (P < 0.01). The content of safflor yellow A in varieties Yutai Honghua, Hefei Honghua, Rucheng Honghua were higher than in others.
CONCLUSION
The effective compound safflor yellow A in C. tinctorius was one of the main quality evaluation criterions. Varieties Yutai Honghua, Hefei Honghua and Rucheng Honghua were good resources.
目的
为建立评价标准、筛选优良品种及开展药材的良好农业规范提供依据。
方法
选取22个代表性的红花品种，在不同生态区域及不同年份进行种植。采用反相高效液相色谱法测定其花冠中红花黄色素A的含量，比较品种间的差异及其遗传稳定性。
结果
红花黄色素A含量范围为0.70% - 1.85%，品种间存在差异（P < 0.01）。鱼台红花、合肥红花、汝城红花中红花黄色素A的含量高于其他品种。
结论
红花中的有效成分红花黄色素A是主要的质量评价标准之一。鱼台红花、合肥红花和汝城红花是优良资源。
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