Tang Xu-li, Liu Jing, Li Guo-qiang, Zhong Hui-min, Qiu Pei-ju, Zhang Jing
Ocean University of China, Qingdao 266003, China.
Zhong Yao Cai. 2007 Aug;30(8):923-9.
To establish the HPLC-fingerprint of the water-soluble constituents of Carthamus tinctorius.
18 samples of Carthamus tinctorius from different producing areas were determined by Agilent 1100 DAD-HPLC under the chromatographic conditions: column by SinoChrom ODS-BP (250 mm x 4.6 mm, 5 microm), methanol-0.7% H3PO4 water with gradient elution, column temperature 30 degrees C, flow rate by 1 ml/min, wavelength 280 nm, and inject volume 20 microl.
The HPLC-fingerprint of the water-soluble constituents of Cartharnus tinctorius was established on the basis of 10 bitch of drugs from Xinjiang according to SPSS analysis.
A reliable method is provided for the quality identification of Carthamus tinctorius.
建立红花水溶性成分的高效液相色谱指纹图谱。
采用安捷伦1100二极管阵列检测器-高效液相色谱仪，对18个不同产地的红花样品进行测定，色谱条件为：色谱柱为中慧ODS-BP（250mm×4.6mm，5μm），以甲醇-0.7%磷酸水为流动相进行梯度洗脱，柱温30℃，流速1ml/min，检测波长280nm，进样量20μl。
根据SPSS分析，以新疆产10批药材为基础建立了红花水溶性成分的高效液相色谱指纹图谱。
为红花的质量鉴定提供了可靠方法。
