[高效液相色谱法对复方板蓝根注射液的指纹图谱分析]
[Fingerprint analysis of composite Folii Isatidis injection by HPLC].
作者信息
Liu Rui, Liu Zhi-gang, Li Lei, Ren Chong, Yuan Bo, Li Fa-mei
机构信息
School of Pharmacy, Shenyang Pharmaceutical University, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(13):1059-62.
OBJECTIVE
To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC.
METHOD
The separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL x min(-1). The UV detection was set at 254 nm.
RESULT
A standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection, with 20 common peaks and a similarity threshold of 9.0 established.
CONCLUSION
This method was accurate, repeatable and useful for the quality control of composite Folii Isatidis injection.
目的
采用高效液相色谱法建立复方大青叶注射液的色谱指纹图谱。
方法
采用 Diamonsil C18 柱，以甲醇 - 水 - 磷酸为流动相进行梯度洗脱，流速为 1.0 mL·min⁻¹，紫外检测波长为 254 nm。
结果
建立了复方大青叶注射液的高效液相色谱指纹图谱标准操作规程，确定了 20 个共有峰，相似度阈值为 0.90。
结论
该方法准确、重复性好，可用于复方大青叶注射液的质量控制。
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