[气滞胃痛颗粒全时多波长融合指纹图谱及多成分定量分析研究]

[Study on all-time multi-wavelength fusion fingerprint of Qizhiweitong granules and multi-component quantitative analysis].

作者信息

Yao Dong, Meng Xian-Sheng, Wang Shuai, Bao Yong-Rui, Pan Ying, Han Ling

机构信息

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1513-7.

PMID:23947127

Abstract

OBJECTIVE

To control the quality of Qizhiweitong granules with the all-time multi-wavelength fusion fingerprint quantification as the major technique.

METHOD

Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) chromatographic column was adopted, with 0.02% formic acid water-acetonitrile as the mobile phase for linear gradient elution. The flow rate was 1 mL x min(-1), column temperature was 30 degrees C, and detector wavelength was 230, 254, 283 nm. Matlab was adopted for all-time multiple-wavelength fusion for data in dif format.

RESULT

A good relationship was shown for albiflorin in 56.5-452 mg x L(-1) (r = 0.999 8), paeoniflorin in 107-856 mg x L(-1) (r = 0.999 8), licorice glycoside in 73.4-687 mg x L(-1) (r = 0.999 8), naringin in 109-872 mg x L(-1) (r = 0.999 8), neohesperidin in 48.0-384 mg L(-1) (r = 0.999 8), and glycyrrhizic acid in 38.6-308 mg x L(-1) (r = 0.999 8), with recoveries of 0.999 8.

CONCLUSION

The method is simple, accurate and highly reproducible, and can provide basis for quality control of Qizhiweitong granules.

摘要

目的

以全时多波长融合指纹图谱定量法为主要技术控制气滞胃痛颗粒的质量。

方法

采用Agilent TC-C18（4.6 mm×250 mm，5 µm）色谱柱，以0.02%甲酸水-乙腈为流动相进行线性梯度洗脱。流速为1 mL·min⁻¹，柱温为30℃，检测波长为230、254、283 nm。采用Matlab对dif格式的数据进行全时多波长融合。

结果

芍药苷在56.5 - 452 mg·L⁻¹范围内线性关系良好（r = 0.999 8），芍药内酯苷在107 - 856 mg·L⁻¹范围内（r = 0.999 8），甘草苷在73.4 - 687 mg·L⁻¹范围内（r = 0.999 8），柚皮苷在109 - 872 mg·L⁻¹范围内（r = 0.999 8），新橙皮苷在48.0 - 384 mg·L⁻¹范围内（r = 0.999 8），甘草酸在38.6 - 308 mg·L⁻¹范围内（r = 0.999 8），回收率均为0.999 8。