Huang Lan, Chen Hui-Ling, Li Ling-Ling
Xiamen Institute for Drugs Control, Xiamen 361012, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jul;38(13):2114-7.
To simultaneously determine paeoniflorin, ferulic acid, prim-O-glucosylcimifugin and 4'-O-beta-glucopyranosyl-5-O-methylvisamminol in Zhengtian pills. In the test, Insertil ODS-C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-0.05% phosphoric acid solution as the mobile phase for gradient elution. The flow rate was 1.0 mL x min(-1), the column temperature was 30 degrees C and the detection wavelength was 230 nm. According to the results of the test, paeoniflorin, ferulic acid, prim-O-glucosylcimifugin and 4'-O-beta-glucopyranosyl-5-O-methylvisamminol showed good linear relations between peak areas and sample sizes in 11.37-170.5, 2.188-32.82, 2.896-43.44, and 3.000-45.00 mg x L(-1) (r = 0.999 9, 0.999 9, 1.000 0, 1.000 0), respectively. The average recoveries (n = 6) were 102.3% (RSD 1.2%), 99.71% (RSD 1.9%), 101.2% (RSD 1.2%), and 99.40% (RSD 2.4%), respectively. The above four components were determined in five batches of samples by using the established method, with satisfactory results. The method was so simple, accurate and highly reproducible that it could be used for quality control of the four components in Zhengtian pills.
同时测定正天丸中芍药苷、阿魏酸、升麻苷和5-O-甲基维斯阿米醇苷的含量。试验采用Insertil ODS-C18柱（4.6 mm×250 mm，5μm），以乙腈-0.05%磷酸溶液为流动相进行梯度洗脱。流速为1.0 mL·min-1，柱温为30℃，检测波长为230 nm。试验结果表明，芍药苷、阿魏酸、升麻苷和5-O-甲基维斯阿米醇苷在11.37 - 170.5、2.188 - 32.82、2.896 - 43.44和3.000 - 45.00 mg·L-1范围内峰面积与进样量呈良好的线性关系（r分别为0.999 9、0.999 9、1.000 0、1.000 0）。平均回收率（n = 6）分别为102.3%（RSD 1.2%）、99.71%（RSD 1.9%）、101.2%（RSD 1.2%）和99.40%（RSD 2.4%）。采用所建立的方法对5批样品中的上述4种成分进行测定，结果满意。该方法简便、准确、重现性好，可用于正天丸中这4种成分的质量控制。
