Si Nan, Yang Jian, Bian Bao-lin
Institute of Chinese Material Medica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(11):884-6.
To compare the solubilities of ephedrine and d-pseudo-ephdrine in the compound of traditional Chinese drugs--Maxing Shigan decoction with and without ultramicro-pulverizations using a RP-HPLC procedure.
The analytical column was a YMC ODS-C18(4.6 mm x 150 mm,4 microm). A mixture of water-acetonitrile-sodium dodecyl sulfate-phosphorous acid (650:350:5:1) was used as the mobile phase at flow rate of 1.5 mL x min(-1). The column temperature was 50 degrees C and the wavelength was 210 nm.
In this way, ephedrine and d-pseudo-ephedrine of the compound samples can be separated well and showed significant differences before and after the ultramicro-pulverizations.
For the Maxing Shigan decoction, the dissolution rates and solubilites of ephedrine and d-pseudo-ephedrine ultramicro-pulverization were greatly improved.
采用反相高效液相色谱法（RP-HPLC）比较麻黄碱和d-伪麻黄碱在中药复方——麻杏石甘汤及其超微粉碎制剂中的溶解度。
分析柱为YMC ODS-C18（4.6 mm×150 mm，4μm）。以水-乙腈-十二烷基硫酸钠-磷酸（650:350:5:1）混合液为流动相，流速为1.5 mL·min⁻¹。柱温为50℃，检测波长为210 nm。
采用该方法，复方样品中的麻黄碱和d-伪麻黄碱能够得到良好分离，且超微粉碎前后有显著差异。
对于麻杏石甘汤，超微粉碎后麻黄碱和d-伪麻黄碱的溶出速率和溶解度有显著提高。
