Hou Qian, Guo Mei, Zhang Fan, Zhou Jin-xia, Liu Li-sha
Department of Biology, Gansu College of TCM, Lanzhou 730000, China.
Zhong Yao Cai. 2012 May;35(5):675-8.
To explore and improve the utilization efficiency of Gentiana macrophylla resources.
The germination of Gentiona macrophylla seeds were used as explants to induce calluses; Cultured on various culture media and condition for callus induction and subculture by test; The content of gentiopicroside in callus was determined by HPLC.
The optimum medium for callus was MS +2,4-D (1.0 mg/L) + 6-BA (0.5 mg/L), and MS +2,4-D (1.0 mg/L) +6-BA (0.5 mg/L) + NAA (0.5 mg/L) was the optimum for subculture,the optimum time of subculture was 30 d; The accumulated gentiopicroside reached the highest level with 0.78 mg/g DW when entered 28-30 d.
The optimized culture method of callus of Gentiana. macrophylla is obtained; The growth curve of callus of Gentiona macrophylla turns on "S" appearance and the accumulated gentiopicroside increases along with the callus appreciation.
探索并提高秦艽资源的利用效率。
以秦艽种子萌发苗为外植体诱导愈伤组织；通过试验在不同培养基及条件下进行愈伤组织的诱导和继代培养；采用高效液相色谱法测定愈伤组织中龙胆苦苷的含量。
愈伤组织诱导的最佳培养基为MS+2,4 - D（1.0毫克/升）+6 - BA（0.5毫克/升），继代培养的最佳培养基为MS+2,4 - D（1.0毫克/升）+6 - BA（0.5毫克/升）+NAA（0.5毫克/升），最佳继代时间为30天；培养28 - 30天时，龙胆苦苷积累量达到最高，为0.78毫克/克干重。
获得了秦艽愈伤组织的优化培养方法；秦艽愈伤组织生长曲线呈“S”形，龙胆苦苷积累量随愈伤组织增值而增加。
