Wang Dong-mei, Liu Chao-shen, Yang De-po
Laboratory of Pharmacognosy and Natural Medicinal Chemistry, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China.
Zhongguo Zhong Yao Za Zhi. 2006 May;31(10):800-4.
To establish the quality control method of the extract enriched hypericins and flavonoids (HFEE) prepared from Hypericum perforatum by means of HPLC fingerprint analysis, and to evaluate the validity, stability of within-day and between-day of the preparing process of HFEE as well as the influence of antioxidant and the herbal source to the quality of HFEE.
HPLC-UV-MS was employed to the qualitative and quantitative analyses of HFEE.
Ten peaks on the HPLC fingerprint of HFEE were indicated, and eight compounds of them had been identified. With respect of the preparing process of HFEE, the validity and stability were significantly observed. There was almost no effect observed on the quality of HFEE whether or not adding the antioxidant during the process. However, different parts of the plant collected as the materials could significantly affect the quality of HFEE.
The method established in the present study is convenient, reliable, and could be used for the quality control of the extract of H. perforatum and for the monitor and control of the preparing process of the extract.
通过高效液相色谱指纹图谱分析建立贯叶连翘富集金丝桃素和黄酮类提取物（HFEE）的质量控制方法，并评价HFEE制备过程日内和日间的有效性、稳定性以及抗氧化剂和药材来源对HFEE质量的影响。
采用高效液相色谱-紫外-质谱联用技术对HFEE进行定性和定量分析。
指出了HFEE高效液相色谱指纹图谱上的10个峰，其中8种化合物已被鉴定。就HFEE的制备过程而言，显著观察到其有效性和稳定性。在该过程中添加或不添加抗氧化剂对HFEE的质量几乎没有影响。然而，作为原料采集的植物不同部位会显著影响HFEE的质量。
本研究建立的方法简便、可靠，可用于贯叶连翘提取物的质量控制以及该提取物制备过程的监测与控制。
