通过5-氨甲酰基乙烯基-2'-脱氧胞苷实现DNA的高选择性和高灵敏度模板导向光连接

Highly selective and sensitive template-directed photoligation of DNA via 5-carbamoylvinyl-2'-deoxycytidine.

作者信息

Yoshimura Yoshinaga, Okamura Daisuke, Ogino Masayuki, Fujimoto Kenzo

机构信息

School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

出版信息

Org Lett. 2006 Oct 26;8(22):5049-51. doi: 10.1021/ol0619462.

DOI:10.1021/ol0619462

PMID:17048840

Abstract

We describe a highly efficient template-directed photoligation of oligodeoxynucleotides (ODNs) through 5-carbamoylvinyl-2'-deoxycytidine ((CV)C). When an ODN containing (CV)C at the 5' end was photoirradiated with an ODN containing a pyrimidine base at the 3' end in the presence of template DNA, efficient photoligation was observed without any byproduct formation. Single nucleotide differences can be successfully distinguished by using photoligation-based DNA chip assay. [structure: see text]

摘要

我们描述了一种通过5-氨甲酰乙烯基-2'-脱氧胞苷（(CV)C）实现的高效模板导向的寡脱氧核苷酸（ODN）光连接。当在模板DNA存在的情况下，对5'端含有(CV)C的ODN与3'端含有嘧啶碱基的ODN进行光照射时，可观察到高效的光连接，且无任何副产物生成。通过基于光连接的DNA芯片检测能够成功区分单核苷酸差异。[结构：见正文]