基于金纳米颗粒的荧光共振能量转移分析法用于DNA切割检测。

Gold nanoparticle based FRET assay for the detection of DNA cleavage.

作者信息

Ray Paresh Chandra, Fortner Angela, Darbha Gopala Krishna

出版信息

J Phys Chem B. 2006 Oct 26;110(42):20745-8. doi: 10.1021/jp065121l.

Abstract

We report gold nanoparticle based FRET assay to monitor the cleavage of DNA by nucleases. Fluorescence signal enhancement is observed by a factor of 120 after the cleavage reaction in the presence of S1 nuclease. The mechanism of distant dependent fluorescence quenching has been discussed. Our experimental results on distance dependent fluorescence quenching match quite well with theoretical findings obtained from the fluorescence quenching model by Gersten and Nitzan (Surf. Sci. 1985, 158, 165). Our experimental observation paradigm for the design of optical based molecular ruler strategies at distances more than double the distances achievable using traditional dipole-dipole Columbic energy transfer based methods.

摘要

我们报道了基于金纳米颗粒的荧光共振能量转移（FRET）分析方法，用于监测核酸酶对DNA的切割。在S1核酸酶存在的情况下进行切割反应后，荧光信号增强了120倍。文中讨论了距离依赖性荧光猝灭的机制。我们关于距离依赖性荧光猝灭的实验结果与Gersten和Nitzan（《表面科学》，1985年，第158卷，第165页）的荧光猝灭模型所得出的理论结果相当吻合。我们用于设计光学分子尺策略的实验观察范式所适用的距离，比使用传统基于偶极 - 偶极库仑能量转移方法所能达到的距离多出一倍以上。