State Key Laboratory of Metal Matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Sensors (Basel). 2013 May 3;13(5):5749-56. doi: 10.3390/s130505749.
An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as "bridges" to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
开发了一种通过使用链霉亲和素包覆的金纳米粒子（StAuNPs）作为高效猝灭剂来选择性和高效地检测单链 DNA 的方法。成功检测的核心概念是结合链霉亲和素-生物素相互作用与特定探针-靶 DNA 杂交。生物素标记的探针 DNA 作为“桥梁”将 Cy5 标记的靶标带到颗粒表面，荧光染料可以被 StAuPNs 快速有效地猝灭。通过测量 Cy5 的光致发光强度的变化，实现了 DNA 杂交的高效、选择性和反向检测。该方法为生物分子的简单快速检测开辟了新途径。
