Tomek W, Buri J, Vallon R, Schmid H P
Biologisches Institut, Universität Stuttgart, F.R.G.
J Chromatogr. 1990 Nov 23;521(2):221-9. doi: 10.1016/0021-9673(90)85046-x.
Prosomes, cytoplasmatic ribonucleoprotein complexes containing small ribonucleic acid (19S small cytoplasmic RNPs), are ubiquitous in eukaryotic organisms. A new method for the preparation of prosomes in large amounts, starting with ca. 2 kg of calf's liver, is described. A combination of centrifugation and low- and high-pressure chromatography was used to purify intact particles. An alternative purification of prosomes with Solanum tuberosum agglutinin bound to divinyl sulphone-activated agarose is discussed. Calf's liver prosomes have a similar protein composition and RNA content to prosomes isolated from other tissues.
原核小体是含有小核糖核酸（19S小细胞质核糖核蛋白）的细胞质核糖核蛋白复合体，在真核生物中普遍存在。本文描述了一种从约2千克小牛肝脏开始大量制备原核小体的新方法。采用离心与低压及高压色谱相结合的方法来纯化完整颗粒。文中还讨论了用与二乙烯砜活化琼脂糖结合的马铃薯凝集素对原核小体进行另一种纯化的方法。小牛肝脏原核小体的蛋白质组成和RNA含量与从其他组织分离的原核小体相似。
