Visick Karen L, Ruby Edward G
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA.
Curr Opin Microbiol. 2006 Dec;9(6):632-8. doi: 10.1016/j.mib.2006.10.001. Epub 2006 Oct 16.
The association of Vibrio fischeri and Euprymna scolopes provides insights into traits essential for symbiosis, and the signals and pathways of bacteria-induced host development. Recent studies have identified important bacterial colonization factors, including those involved in motility, bioluminescence and biofilm formation. Surprising links between symbiosis and pathogenesis have been revealed through discoveries that nitric oxide is a component of the host defense, and that V. fischeri uses a cytotoxin-like molecule to induce host development. Technological advances in this system include the genome sequence of V. fischeri, an expressed sequence tagged library for E. scolopes and the availability of dual-fluorescence markers and confocal microscopy to probe symbiotic structures and the dynamics of colonization.
费氏弧菌与夏威夷短尾乌贼的共生关系为共生所需的特性以及细菌诱导宿主发育的信号和途径提供了见解。最近的研究已经确定了重要的细菌定殖因子，包括那些参与运动性、生物发光和生物膜形成的因子。通过发现一氧化氮是宿主防御的一个组成部分，以及费氏弧菌使用一种细胞毒素样分子来诱导宿主发育，揭示了共生与发病机制之间令人惊讶的联系。该系统的技术进步包括费氏弧菌的基因组序列、夏威夷短尾乌贼的表达序列标签文库，以及用于探测共生结构和定殖动态的双荧光标记和共聚焦显微镜的可用性。
