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[山梨醇对采用比色法测定酮胺的影响]

[The effect of sorbitol on the determination of keto amines using a colorimetric method].

作者信息

Kovaleva G G, Gorodetskiĭ V K

出版信息

Lab Delo. 1990(11):50-2.

PMID:1704959
Abstract

The presence of sorbitol in a concentration 11 mg/ml in a sample reduces by 68 percent the values of fructose glycine measured by colorimetry with thiobarbituric acid. Such sorbitol concentration has no effect on furfural production and on 5-hydroxymethylfurfural reaction with thiobarbituric acid. Measurements of glycolyzed hemoglobin have demonstrated that sorbitol in concentration 11 mg/ml completely inhibits hydrolysis. This fact should be borne in mind when measuring blood glycosylated hemoglobin by colorimetry with thiobarbituric acid and exclude sorbitol from patients' rations before measurements.

摘要

样品中浓度为11毫克/毫升的山梨醇使通过硫代巴比妥酸比色法测量的果糖甘氨酸值降低了68%。这种山梨醇浓度对糠醛生成以及5-羟甲基糠醛与硫代巴比妥酸的反应没有影响。糖酵解血红蛋白的测量表明,浓度为11毫克/毫升的山梨醇完全抑制水解。在用硫代巴比妥酸比色法测量血糖化血红蛋白时应牢记这一事实,并在测量前从患者饮食中排除山梨醇。

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